Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa
AUTOR(ES)
Oliveira, GH de, Berchieri Júnior, Â, Montassier, HJ, Fernandes, AC
FONTE
Revista Brasileira de Ciência Avícola
DATA DE PUBLICAÇÃO
2004-06
RESUMO
This study was done to assess an enzyme-linked immunosorbent assay (ELISA) to detect chicken serological response against Salmonella enterica serotypes Gallinarum and Pullorum. The assays have used soluble bacterial proteins of Salmonella Gallinarum strain 9 (AgSG) as detecting antigen and peroxidase and alkaline phosphatase conjugates. According to the results, the antigen, sera and conjugate concentrations were optimized. In addition, the assay using alkaline phosphatase and peroxidase conjugates was helpful to distinguish positive serological reaction to serotypes Gallinarum and Pullorum from Enteritidis.
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