Assimilation of Nitrogen from Nitrite and Trinitrotoluene in Pseudomonas putida JLR11

AUTOR(ES)

Caballero, Antonio

FONTE

American Society for Microbiology

RESUMO

Pseudomonas putida JLR11 releases nitrogen from the 2,4,6-trinitrotoluene (TNT) ring as nitrite or ammonium. These processes can occur simultaneously, as shown by the observation that a nasB mutant impaired in the reduction of nitrite to ammonium grew at a slower rate than the parental strain. Nitrogen from TNT is assimilated via the glutamine syntethase-glutamate synthase (GS-GOGAT) pathway, as evidenced by the inability of GOGAT mutants to use TNT. This pathway is also used to assimilate ammonium from reduced nitrate and nitrite. Three mutants that had insertions in ntrC, nasT, and cnmA, which encode regulatory proteins, failed to grow on nitrite but grew on TNT, although slower than the wild type.

Documentos Relacionados

• Respiration of 2,4,6-Trinitrotoluene by Pseudomonas sp. Strain JLR11
• Nitrite Assimilation and Amino Nitrogen Synthesis in Isolated Spinach Chloroplasts 1
• RELATIONSHIP OF NITRITE AND HYDROXYLAMINE REDUCTASES TO NITRATE ASSIMILATION AND NITROGEN FIXATION IN AZOTOBACTER AGILE1
• Purification and properties of formylglutamate amidohydrolase from Pseudomonas putida.
• Distribution of nitrogen-13 from labeled nitrate and nitrite in germfree and conventional-flora rats.