Asymmetric expression in somites of cytotactin and its proteoglycan ligand is correlated with neural crest cell distribution.
AUTOR(ES)
Tan, S S
RESUMO
The development of the vertebrate neural crest presents a particularly challenging problem in pattern formation. Several studies have revealed that a population of neural crest cells penetrates the sclerotomal mesenchyme of the somite only in its rostral half. In a search for molecular correlates of this pattern, we have observed that cytotactin and a chondroitin sulfate proteoglycan, two interactive extracellular matrix molecules, show a specialized distribution within the sclerotome. Cytotactin was localized in the rostral half of the sclerotome at about the time of neural crest cell invasion. The proteoglycan was initially diffuse throughout the sclerotome but became restricted to the caudal half after the appearance of cytotactin and invasion of neural crest cells in the rostral half. These distributions were crest cell-independent; they occurred on the same schedule even when all crest cells were removed by surgical extirpation of the neural tube. Furthermore, in tissue culture, somite cells synthesized high levels of both molecules. In vitro, crest cells rounded up in the presence of these molecules and cell migration assays revealed that neither cytotactin nor proteoglycan alone was as good a substratum for crest cell migration as fibronectin. In combination with fibronectin, however, cytotactin or proteoglycan only restricted cell movement but did not prevent it. Taken together, these observations support the hypothesis that cytotactin and the chondroitin sulfate proteoglycan may contribute to pattern formation during embryogenesis by means of their site-restricted distribution, their ability to alter migration on other substrates such as fibronectin, and their ability to induce cell-surface modulation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=299459Documentos Relacionados
- Cytotactin expression in somites after dorsal neural tube and neural crest ablation in chicken embryos.
- Emigration of neural crest cells from macaque optic vesicles is correlated with discontinuities in its basement membrane.
- A proteoglycan with HNK-1 antigenic determinants is a neuron-associated ligand for cytotactin.
- The microenvironment created by grafting rostral half-somites is mitogenic for neural crest cells.
- The neural crest stem cells: control of neural crest cell fate and plasticity by endothelin-3