ATP-dependent Ca2+ uptake into plant membrane vesicles

AUTOR(ES)

Gross, Joachim

RESUMO

Membrane vesicles were extracted from etiolated and light-grown plants, a plant cell suspension culture, and an alga. Upon addition of ATP and Mg2+, active Ca2+ uptake into the vesicles against a concentration gradient was shown. The dependence of this uptake on ATP and Mg2+ concentrations, pH, and temperature is described. In the absence of oxalate, equilibrium between Ca2+ uptake and efflux was reached after about 30 min. In the presence of oxalate, Ca2+ accumulation continued for at least 120 min. Ca2+ efflux from preloaded vesicles did not depend on ATP. Addition of the ionophores A23187 and Ro 20-0006/006 caused an immediate release of accumulated free Ca2+. ATP-dependent Ca2+ uptake was not inhibited by 10 μM oligomycin.

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