ATP enhances the binding of simian virus 40 large T antigen to the origin of replication.
AUTOR(ES)
Deb, S P
RESUMO
Simian virus 40 large T antigen initiates DNA replication by binding to the origin of replication. We examined the binding of T antigen to origin regions I, II, and III under conditions designed for efficient in vitro replication functions. We found that 4 mM ATP enhanced the binding of T antigen to regions I and II of the origin DNA by 4- to 20-fold. DNase-footprinting and fragment assays showed that ATP extended the DNase protection domain of T antigen bound to region II by 5 to 10 base pairs at both ends of the core origin of replication. This alteration suggests a change in the conformation of T antigen, bound DNA, or both.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=255975Documentos Relacionados
- ATP stimulates the binding of simian virus 40 (SV40) large tumor antigen to the SV40 origin of replication.
- The T-antigen-binding domain of the simian virus 40 core origin of replication.
- T antigen binds to simian virus 40 DNA at the origin of replication.
- Simian virus 40 (SV40) DNA replication: SV40 large T antigen unwinds DNA containing the SV40 origin of replication.
- Simian virus 40 origin DNA-binding domain on large T antigen.