Automatic Determination of Bacterioplankton Biomass by Image Analysis †
AUTOR(ES)
Bjørnsen, Peter Koefoed
RESUMO
Image analysis was applied to epifluorescense microscopy of acridine orange-stained plankton samples. A program was developed for discrimination and binary segmentation of digitized video images, taken by an ultrasensitive video camera mounted on the microscope. Cell volumes were estimated from area and perimeter of the objects in the binary image. The program was tested on fluorescent latex beads of known diameters. Biovolumes measured by image analysis were compared with directly determined carbon biomasses in batch cultures of estuarine and freshwater bacterioplankton. This calibration revealed an empirical conversion factor from biovolume to biomass of 0.35 pg of C μm−3 (± 0.03 95% confidence limit). The deviation of this value from the normally used conversion factors of 0.086 to 0.121 pg of C μm−3 is discussed. The described system was capable of measuring 250 cells within 10 min, providing estimates of cell number, mean cell volume, and biovolume with a precision of 5%.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=239044Documentos Relacionados
- Relationships between Biovolume and Biomass of Naturally Derived Marine Bacterioplankton †
- Efficacy of Phospholipid Analysis in Determining Microbial Biomass in Sediments †
- Determination of Biomass by Ultrasonic Measurements
- Automatic flow analysis procedure for the determination of bromide in L-alanine by chemiluminescence detection
- Determination of Bacterial Cell Dry Mass by Transmission Electron Microscopy and Densitometric Image Analysis
