Autoregulation by tandem promoters of the Salmonella typhimurium LT2 metJ gene.
AUTOR(ES)
Urbanowski, M L
RESUMO
Regulation of the Salmonella typhimurium metJ gene was examined by measuring beta-galactosidase activity in Escherichia coli strains lysogenic for a phage carrying a metJ-lacZ gene fusion. The results indicated that the metJ gene is regulated by its own gene product and by methionine supplementation to the growth medium. This autoregulatory mechanism involved two tandem promoters, pJ1 and pJ2, separated by approximately 65 base pairs. Deletion analysis permitted the assessment of the activity of promoters pJ1 and pJ2 individually. Promoter Pj1 was negatively regulated by the metJ gene product and by methionine. Although Pj2 regulation remained unclear, evidence is presented which suggests that it is not negatively regulated like pJ1.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=214491Documentos Relacionados
- Nucleotide sequence and biochemical characterization of the metJ gene from Salmonella typhimurium LT2.
- Physical map of Salmonella typhimurium LT2 DNA in the vicinity of the proA gene.
- Avirulence of LT2 strains of Salmonella typhimurium results from a defective rpoS gene.
- New Antibiotic Substance Produced by Salmonella typhimurium LT2
- The Salmonella typhimurium LT2 uvrD gene is regulated by the lexA gene product.
