Aux/IAA proteins repress expression of reporter genes containing natural and highly active synthetic auxin response elements.
AUTOR(ES)
Ulmasov, T
RESUMO
A highly active synthetic auxin response element (AuxRE), referred to as DR5, was created by performing site-directed mutations in a natural composite AuxRE found in the soybean GH3 promoter. DR5 consisted of tandem direct repeats of 11 bp that included the auxin-responsive TGTCTC element. The DR5 AuxRE showed greater auxin responsiveness than a natural composite AuxRE and the GH3 promoter when assayed by transient expression in carrot protoplasts or in stably transformed Arabidopsis seedlings, and it provides a useful reporter gene for studying auxin-responsive transcription in wild-type plants and mutants. An auxin response transcription factor, ARF1, bound with specificity to the DR5 AuxRE in vitro and interacted with Aux/IAA proteins in a yeast two-hybrid system. Cotransfection experiments with natural and synthetic AuxRE reporter genes and effector genes encoding Aux/IAA proteins showed that overexpression of Aux/IAA proteins in carrot protoplasts resulted in specific repression of TGTCTC AuxRE reporter gene expression.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=157050Documentos Relacionados
- Auxin modulates the degradation rate of Aux/IAA proteins
- AUX/IAA Proteins Are Active Repressors, and Their Stability and Activity Are Modulated by Auxin
- Protein–protein interactions among the Aux/IAA proteins
- Aux/IAA Proteins Contain a Potent Transcriptional Repression Domain
- Aux/IAA Proteins Are Phosphorylated by Phytochrome in Vitro1