Avaliação da toxicidade induzida pelos componentes do radiofármaco 99m Tc-MDP em cepa de E. coli AB1157 e células eucarióticas de ratos e humanos / Evaluation of toxicity induced by 99mtc-MDP radiopharmaceutical componentsin E. coli AB 1157 and human and rats eukaryotic cells

AUTOR(ES)

Michelle Pinheiro Rodrigues

DATA DE PUBLICAÇÃO

2008

RESUMO

Alive cells are constantly threated by chemical and physical agents that can generate DNA damage. Here, the studied agent was stannous chloride (SnCl2), a 99mTc reducing agent employed in nuclear medicine. This salt can produce lesions through generation of reactive oxygen species (ROS) as H2O2 and OH. These ROS can be the origin of several diseases and cell death by apoptosis or necrosis. In this way it is important the research about the biological effects of this salt and the other substances composing the radiopharmaceuticals used in nuclear medicine.The aim of this work was to evaluate, in different biological systems, the stannous chloride toxic potentiality, associated or not to 99mTc-MDP radiopharmaceutical as well as the other kit components. Alkaline electrophoresis agarosis gel of E. coli AB 1157 to evaluate the genotoxicity in prokariotic cells; Comet assay in Wistar rats total blood to evaluate the genotoxicity in eukaryotic cells; Micronucleous assay in Wistar rats bone marrow cells to verify the aneugenic and clastogenic effects; Comet assay in human in peripherical total blood and mononuclear cells to evaluate the genotoxicity; Trypan blue viability assay and flow cytometry to evaluate citotoxicity in PBMC; Micronucleous assay in human lymphocyte cells to verify the aneugenic and clastogenic effects; SnCl2 and MDP when isolated induced breaks in E. coli genomic DNA. But, when used in an associated way it was observed an atenuation of the breaks number. 99mTc-MDP was not genotoxic, clastogenic nor aneugenic in Wistar rats In ex vivo human total blood, SnCl2 presented a dose-response effect In ex vivo PBMC; 99mTc-MDP induced a cellular viability reduction and presented genotoxic but not clastogenic or aneugenic effects.

ASSUNTO(S)

stannous chloride radiofármaco sistemas biológicos escherichia coli. eukariotic cells biological systems genotoxicidade escherichia coli estanho genotoxicity células eucarióticas anatomia patologica e patologia clinica radiopharmaceutical

Documentos Relacionados

• Avaliação da relevância das imagens simultâneas de fusão: SPECT com 99m Tc-MDP e Tomografia Computadorizada aplicada no diagnóstico das Disfunções Temporomandibulares.
• Phenotypic variations in strain AB1157 cultivars of Escherichia coli from different sources.
• Effect of antimalarial drugs on the bioavailability of the methylenediphosphonic acid labeled with technetium99m (99mTc-MDP) in Wistar rats
• Resultados discordantes em cintilografias das paratireóides realizadas com tetrofosmin-99mTc e com sestamibi-99mTc
• Estudos de técnicas de concentração da  atividade de 99mTc eluído de geradores de 99Mo/99mTc tipo gel