Avaliação de um novo kit (TF-TEST) nacional destinado ao diagnostico de enteroparasitoses em amostras fecais

AUTOR(ES)
DATA DE PUBLICAÇÃO

2004

RESUMO

Human intestinal parasitoses constitute a serious public health problem either in developing or developed countries. Those enteroparasitoses could be attributed to the increase of travelers around endemic areas and mainly due to immunocompromised patients. In order to obtain sensible results and to have a high chance to find organisms by multiple sample exams, it is necessary at least three serial parasitologycal faecal tests. For these reasons, a practice and non-expensive kit (TF-Test) was developed for being industrialized by a specialized company (Immunoassay Ind. Com. Ltda., Sao Paulo, Brazil). This kit makes possible to carry out three different faecal samples each other day. The three faecal samples are separately collected into tubes containing a preservative solution. Laboratory evaluation is done in just one step by using a pool of the three faecal samples. Mixture mass is passing by a double filter and then centrifuged for concentrating the mixture. Thus, the present study reports about evaluations of a prototype kit developed into two different phases: first phase was a six months stage inside developer laboratory and, the second one was a two years stage involving six different collaborative laboratories. In the first phase, parasitologycal exams using TF-Test was carried out in 30 patients with intestinal parasites and in 30 patients free of intestinal parasites and, it was significantly more sensible (96,6%, p<0,05) compared with conventional techniques as Coprotest, StillQuick e Parasep or with those frequently used as Lutz or Hoffman, Pons &Janer. Furthermore, TF-Test had a high agreement k index (0,920), which was considered as Almost Perfect, in addition to differ from conventional techniques. Thus, focusing in the potential use of TF-Test for diagnosing different intestinal parasites, this work had been continued for developing a better prototype kit of TF-Test. In the second phase, six different specialized laboratories from public or private service had evaluated faecal samples collected from 1.244 patients. Data from those laboratories attested that TF-Test was actually better than other known techniques (Coprotest, Lutz and Lutz, Faust &Cols. and Rugai, Mattos &Brisola), for presenting sensibility range between 82,6% and 100%, which was significantly higher than conventional methods (p <0,05). In four of the six laboratories, k index of TF-Test was classified as Almost Perfect, and in the other two laboratories, the index was considered as Substancial. The high sensibility of TF-Test in addition to its practice and economic aspects shown that TF-Test method is very appropriated for individual diagnosis, population inquires as well as for evaluating therapy programs in the control of intestinal parasitoses

ASSUNTO(S)

não informado.

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