Avaliação do efeito da radiação laser de baixa potência sobre a proliferação de linfócitos humanos tratados com cisplatina

AUTOR(ES)

Renata Lazari Sandoval

DATA DE PUBLICAÇÃO

2007

RESUMO

Low energy laser radiation has been successfully used for the prevention and treatment of oral ulcerations induced by cytotoxic therapies (radiotherapy and chemotherapy), during oncologic treatment. The healing effect of laser radiation is a consequence of its potential to induce cell proliferation, including the cells that participate in tissue repair. In spite of its wide use, its role in the prevention of oral ulcerations is not completely understood, owing to investigational limitations. These limitations exist due to special aspects associated to the oncologic patient, including myelosupression and higher risk of infection. The development of an in vitro model that is capable of reproducing in vivo situations is mandatory to evaluate the effects caused by laser irradiation and to elucidate its mechanisms of action. With this purpose, we developed a model that was able to reproduce cytotoxicity caused by chemotherapeutic agents, cisplatin in this case, as well as interindividual response variations, stimulatory effect on cell proliferation and cytoprotection induced by low energy laser radiation. In the present model, lymphocyte proliferation was the biological marker used to study the aforementioned circumstances. Laser radiation increased the lymphocyte proliferative response and promoted cytoprotection when the cells were exposed to cisplatin. It was not possible to show any relation between energy density of laser radiation and the obtained proliferative responses because of interindividual variations. These variations were also noted in the cytotoxicity assay induced by cisplatin.

ASSUNTO(S)

raios laser oncologia medicina mucosa bucal medicina oral odontologia

Documentos Relacionados

• Efeitos da terapia laser de baixa potência sobre o reparo ósseo: avaliação do osso submetido à radioterapia e do efeito sistêmico
• Análise comparativa dos efeitos do ultrassom terapêutico e laser de baixa potência sobre a proliferação de células musculares durante a diferenciação celular
• Avaliação funcional da nocicepção do joelho de ratos tratada com laser de baixa potência e natação
• Avaliação da aplicação do laser de baixa potência com ou sem a associação de antimicrobiana em radiomucosite oral
• Efeito do latex de Euphorbia milii sobre a proliferação de linfócitos induzida por mitogénio