Avoidance of sulfur loss during ammonia treatment of oligonucleotide phosphorothioates.
AUTOR(ES)
Reese, C B
RESUMO
Sulfur loss during the unblocking of phosphorothioate analogues of oligonucleotides with concentrated aqueous ammonia can be completely suppressed by the addition of 2-mercaptoethanol.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=146813Documentos Relacionados
- Long-term treatment of human immunodeficiency virus-infected cells with antisense oligonucleotide phosphorothioates.
- Stability of oligodeoxynucleoside phosphorodithioates and phosphorothioates in aqueous ammonia.
- Sequencing of oligonucleotide phosphorothioates based on solid-supported desulfurization.
- Phosphorothioate-modified oligodeoxyribonucleotides. III. NMR and UV spectroscopic studies of the Rp-Rp, Sp-Sp, and Rp-Sp duplexes, [d(GGSAATTCC)]2, derived from diastereomeric O-ethyl phosphorothioates.
- Weight loss dynamics during combined fluoxetine and olanzapine treatment