Axin localizes to the centrosome and is involved in microtubule nucleation

AUTOR(ES)

Fumoto, Katsumi

FONTE

Nature Publishing Group

RESUMO

Axin is known to have an important role in the degradation of β-catenin in the Wnt pathway. Here, we reveal a new function of Axin at the centrosome. Axin was localized to the centrosome in various cell lines and formed a complex with γ-tubulin. Knockdown of Axin reduced the localization of γ-tubulin and γ-tubulin complex protein 2—components of the γ-tubulin ring complex—to the centrosome and the centrosomal microtubule nucleation activity after treatment with nocodazole. These phenotypes could not be rescued by the reduction in the levels of β-catenin. Although the expression of Axin rescued these phenotypes in Axin-knockdown cells, overexpression of Axin2, which is highly homologous to Axin, could not. Axin2 was also localized to the centrosome, but it did not form a complex with γ-tubulin. These results suggest that Axin, but not Axin2, is involved in microtubule nucleation by forming a complex with γ-tubulin at the centrosome.

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