Bacteriophage lambda and plasmid vectors, allowing fusion of cloned genes in each of the three translational phases.
AUTOR(ES)
Charnay, P
RESUMO
We have constructed vectors from bacteriophage lambda and from plasmid pBR322 having a single EcoRI restriction site which is immediately downstream from the lac UV5 promotor. Each vector allows the fusion of a cloned gene to the lac Z gene in a different phase relative to the translation initiation codon of the lac Z gene. These vectors were constructed through modification of the initial EcoRI restriction site by S1 endonuclease treatment and then addition of octadeoxyribonucleotides (EcoRI linkers), which shifted the restriction site by 2 or 4 nucleotides. Used in combination these vectors should allow translation of a cloned gene in any one of the three coding phases. The bacteriophages vectors are certified as B2 (EK2) safety level vectors by the French "recombinaison génétique in vitro" committee (D.G.R.S.T.).
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=342767Documentos Relacionados
- Temporal and pharmacological division of fibroblast cyclooxygenase expression into transcriptional and translational phases.
- Embryonic stem cell gene targeting using bacteriophage lambda vectors generated by phage-plasmid recombination.
- Bronchorrhoea--separation of mucus and serum components in sol and gel phases.
- Construction and characterization of the hybrid bacteriophage lambda Charon vectors for DNA cloning.
- Isolation and characterization of mutations in the bacteriophage lambda terminase genes.
