Bacteriophage T4 late promoters: mapping 5' ends of T4 gene 23 mRNAs.
AUTOR(ES)
Kassavetis, G A
RESUMO
More than thirty 5' ends of RNA molecules which transverse the bacteriophage T4 late gene, gene 23, have been mapped by nuclease protection and reverse transcription, to a 1.7 kilobase pair (kbp) region upstream of gene 23. Most of these 5' ends arise from RNA processing and degradation. Two sites at which RNA synthesis is initiated have been identified by guanylylation of diphosphate- or triphosphate-terminated RNA with vaccinia virion "capping" enzyme and are situated approximately 0.1 and 0.9 kbp upstream of gene 23. At least one other promoter lies more than 1.7 kbp upstream of gene 23, that is, outside of the mapped region.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=553003Documentos Relacionados
- Capped poly(A) leaders of variable lengths at the 5' ends of vaccinia virus late mRNAs.
- A highly sensitive method for mapping the 5' termini of mRNAs.
- Escherichia coli Endoribonucleases Involved in Cleavage of Bacteriophage T4 mRNAs
- Sequence arrangement of the 5' ends of simian virus 40 16S and 19S mRNAs.
- Heterogeneity in the 5' untranslated region of mouse cytochrome cT mRNAs leads to altered translational status of the mRNAs.