Bald mutants of Streptomyces griseus that prematurely undergo key events of sporulation.
AUTOR(ES)
Kwak, J
RESUMO
To identify the structural defects of nonsporulating mutants of Streptomyces griseus, the wild-type strain and class III bald mutants were examined by using transmission electron microscopy, ultrasonic treatment, and fluorescence microscopy after the induction of submerged sporulation by phosphate starvation. In the wild-type strain, submerged sporulation was marked by the relatively synchronous formation of sporogenic hyphae, nucleoid segregation, deposition of sporulation septa, and subsequent thickening of the spore walls during maturation. All of the class III mutants prematurely synthesized sporulation septa and thick spore walls. The class IIIA and C mutants formed sporogenic hyphae earlier than the wild-type strain and underwent nucleoid segregation in parallel with sporulation septum formation. In the class IIIB (bld4) mutant, DNA segregation appeared to be uncoupled from septum formation. The results indicate that the class III mutants are defective in loci that are involved in the regulation of key events of Streptomyces morphogenesis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=178235Documentos Relacionados
- Analysis of a gene that suppresses the morphological defect of bald mutants of Streptomyces griseus.
- The A-factor-binding protein of Streptomyces griseus negatively controls streptomycin production and sporulation.
- Sporulation of Streptomyces griseus in submerged culture.
- Cloning of DNA involved in sporulation of Streptomyces griseus.
- Visualization of Penicillin-Binding Proteins during Sporulation of Streptomyces griseus