Basic Studies for Continuous Production of L-Aspartic Acid by Immobilized Escherichia coli Cells
AUTOR(ES)
Tosa, Tetsuya
RESUMO
By using a column packed with immobilized Escherichia coli cells entrapped in a polyacrylamide gel lattice, conditions for continuous production of L-aspartic acid from ammonium fumarate were investigated. When a solution of 1 M ammonium fumarate (pH 8.5) containing 1 mM Mg2+ was passed through the immobilized cell column at a flow rate of space velocity (SV) = 0.8 at 37 C, the highest rate of reaction was attained. From the column effluents, L-aspartic acid was obtained in good yield. The immobilized cell column was very stable.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=380171Documentos Relacionados
- Immobilization of Escherichia coli Cells Containing Aspartase Activity with Polyurethane and Its Application for l-Aspartic Acid Production
- PANTOTHENATE STUDIES I. : Interference by d-Serine and l-Aspartic Acid with Pantothenate Synthesis in Escherichia coli
- Effect of L-Aspartic Acid and L-Glutamic Acid on Production of L-Proline
- Determination of L-aspartic acid by using the Cu(II)-catalyzed oscillating reaction
- Amino Acid Transport in Suspension-Cultured Plant Cells 1: III. COMMON CARRIER SYSTEM FOR THE UPTAKE OF l-ARGININE, l-ASPARTIC ACID, l-HISTIDINE, l-LEUCINE, AND l-PHENYLALANINE