BIM1 Encodes a Microtubule-binding Protein in Yeast
AUTOR(ES)
Schwartz, Katja
FONTE
The American Society for Cell Biology
RESUMO
A previously uncharacterized yeast gene (YER016w) that we have named BIM1 (binding to microtubules) was obtained from a two-hybrid screen of a yeast cDNA library using as bait the entire coding sequence of TUB1 (encoding α-tubulin). Deletion of BIM1 results in a strong bilateral karyogamy defect, hypersensitivity to benomyl, and aberrant spindle behavior, all phenotypes associated with mutations affecting microtubules in yeast, and inviability at extreme temperatures (i.e., ≥37°C or ≤14°C). Overexpression of BIM1 in wild-type cells is lethal. A fusion of Bim1p with green fluorescent protein that complements the bim1Δ phenotypes allows visualization in vivo of both intranuclear spindles and extranuclear microtubules in otherwise wild-type cells. A bim1 deletion displays synthetic lethality with deletion alleles of bik1, num1, and bub3 as well as a limited subset of tub1 conditional-lethal alleles. A systematic study of 51 tub1 alleles suggests a correlation between specific failure to interact with Bim1p in the two-hybrid assay and synthetic lethality with the bim1Δ allele. The sequence of BIM1 shows substantial similarity to sequences from organisms across the evolutionary spectrum. One of the human homologues, EB1, has been reported previously as binding APC, itself a microtubule-binding protein and the product of a gene implicated in the etiology of human colon cancer.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=25736Documentos Relacionados
- PinX1 Is a Novel Microtubule-binding Protein Essential for Accurate Chromosome Segregation*
- Functional Elements within the Dynein Microtubule-binding Domain
- Dynein and kinesin share an overlapping microtubule-binding site
- In Vivo Dynamics and Differential Microtubule-Binding Activities of MAP65 Proteins1
- KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in C. elegans