Binding Interaction between Tet(M) and the Ribosome: Requirements for Binding
AUTOR(ES)
Dantley, Kathi A.
FONTE
American Society for Microbiology
RESUMO
Tet(M) protein interacts with the protein biosynthesis machinery to render this process resistant to tetracycline by a mechanism which involves release of the antibiotic from the ribosome in a reaction dependent on GTP hydrolysis. To clarify this resistance mechanism further, the interaction of Tet(M) with the ribosome has been examined by using a gel filtration assay with radioactively labelled Tet(M) protein. The presence of GTP and 5′-guanylyl imido diphosphate, but not GDP, promoted Tet(M)-ribosome complex formation. Furthermore, thiostrepton, which inhibits the activities of elongation factor G (EF-G) and EF-Tu by binding to the ribosome, blocks stable Tet(M)-ribosome complex formation. Direct competition experiments show that Tet(M) and EF-G bind to overlapping sites on the ribosome.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=107402Documentos Relacionados
- Nucleotide sequence of the tet(M) gene of Tn916.
- Characterization of the tet(M) determinant of Tn916: evidence for regulation by transcription attenuation.
- Host Mutations (miaA and rpsL) Reduce Tetracycline Resistance Mediated by Tet(O) and Tet(M)
- Genetic Diversity of the tet(M) Gene in Tetracycline-Resistant Clonal Lineages of Streptococcus pneumoniae
- Molecular Characterization of tet(M) Genes in Lactobacillus Isolates from Different Types of Fermented Dry Sausage