Binding of mouse interferon to polynucleotides.
AUTOR(ES)
De Maeyer-Guignard, J
RESUMO
The polynucleotides poly(I) and poly(U), and to a lesser extent poly(G), are capable of desorbing mouse interferon from blue dextran-Sepharose columns, whereas poly(A) andpoly(C) are without effect. When covalently bound to agarose, poly(I), poly(U), and also poly(A) act as potent ligands for purification of interferon by affinity chromatography. Furthermore, poly(I) and poly(U) confer a significant degree of protection to interferon against thermal denaturation. Taken together, these observations point to a direct interaction of interferon with these polymers and suggest that interferon molecules have a polynucleotide attachment site. Possible implications for the concept of interferon induction are discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=431731Documentos Relacionados
- Induction of interferon synthesis by synthetic double-stranded polynucleotides.
- Intercalative and nonintercalative binding of large cationic porphyrin ligands to polynucleotides.
- Is Tb3+ fluorescence enhancement only due to binding to single stranded polynucleotides.
- Derivatization of unprotected polynucleotides.
- A theoretical investigation on the sequence selective binding of adriamycin to double-stranded polynucleotides.