Bioassay Method for Polyene Antibiotics Based on the Measurement of Rubidium Efflux from Rubidium-Loaded Yeast Cells
AUTOR(ES)
Cosgrove, R. F.
RESUMO
A bioassay method for the polyene antibiotics nystatin and amphotericin B is proposed based on the measurement of the efflux of rubidium ions from a rubidium-loaded yeast culture challenged with the antibiotics. For this purpose a major proportion of the intracellular K+ ions in a Saccharomyces cerevisiae culture has been substituted by Rb+ ions. The rubidium leakage is measured by atomic absorption spectrophotometry, and a straight-line, dose-response correlation has been obtained for both antibiotics.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=351913Documentos Relacionados
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