Biochemical and genetic properties of site-specific restriction endonucleases in Bacillus globigii.
AUTOR(ES)
Duncan, C H
RESUMO
Bacillus globigii contains two site-specific endonucleases, BPGLI AND BglI. A rapid technique for selection of mutants deficient in each of these enzymes was developed using sensitivity to infection by bacteriophage SP50 as an indication of the levels of enzyme. Mutants defective in BglI, BglII, and both BglI and BglII retained the wild-type modification phenotype. Genetic and biochemical studies have established that these enzymes are involved in restriction in vivo. Simplified purification procedures for BglI and BglII using these mutants are described.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=222251Documentos Relacionados
- Site-specific methylation: effect on DNA modification methyltransferases and restriction endonucleases
- Effect of site-specific modification on restriction endonucleases and DNA modification methyltransferases.
- Effect of site-specific methylation on restriction endonucleases and DNA modification methyltransferases.
- Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases
- Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases