Biological evaluation of calcium from chicken eggshell (Gallus Gallus, L.) powder in healthly and osteoporosis induced female rats (Rattus norvegicus, Wistar). 2009. 122p Dissertation (Master Science in Food Technology). / Avaliação biológica do cálcio da casca de ovo de galinha (Gallus Gallus, L.) em pó em ratas (Rattus norvegicus, Wistar) saudáveis e com osteoporose induzida.

Juliana dos Santos Vilar

The eggshell, agroindustrial by-product can be considered like a potential source of calcium for human, being one of the ingredients of food multimixtures distributed in Brazil like a strategy to combat malnutrition. The lack of chemical informations about eggshell caused the realization of this work that aimed to develop a technology to obtain a product in a powder form, determine its content of nutritious and evaluate the biological use of its fraction of calcium in healthly female rats and/or osteorosis induced by castration and glucorticoid therapy. After washed in water and immersed in 200 ppm sodium hypochlorite solution for 15 minutes, the shlles were centrifugated to 2.000 rpm (g=9,80), dried with heated air circulation to 55 5C and turned into a powder with a mill, obtained a profit of 98,45 0,70 %. Investigations of total bacterial aerobic counts, mold and yeasts, coliforms 35 C and 45 C and Salmonella spp., showed the efficiency of the process employed. Analytical determinations showed that 100g of this product contained low moisture (1,3490 0,0274g), ash equivalent to 91,9600 0,2218g and low caloric value (30,6823 4,9069 Kcal), originated from 4,3693 0,3977g of proteins, 0,7837 0,0560g of lipídis and 1,5379 0,7029g of total carbohydrates, besides 30.263,72 mg of calcium. Diet for maintenance to adult rats Wistar were prepared (control), in which the source of calcium was substituted by egghell powder and supplemented or not with vitamins D and K (experimentals). Healthy or osteoporosis induced rats, divided into 8 groups received diet and water ad libitum, during 33 days. Morphological, biochemical and hematological parameters were evaluated. Rats of group 7 (without vitamin D) presented femurs significantly more heavy than rats of group 6 (without vitamin K), what could suggest the biggest importance of the vitamin D compared to vitamin K in the process of bone formation. For the kidneys were observed differences between the right and left organ. These organs of group 3 (diet with calcium of the shell) presented average of weights significantly bigger than the other experimental groups. Relate to bone contents of phosphorus, animal of group 6 (diet without vitamin K) were different from the animals of control group, with similar levels to basal group and inferiors to the groups 2 (osteoporosis induced + diet of maintenance), 8 (diet supplemented with calcium of the shell and vitamins D and K) and control group. Relate to bone contents of magnesium and calcium, the animals of the groups 2 and 8 did not present significant differences compared to control group, and the animals of group 5 (diet without vitamins D and K) presented less contents of calcium in their bones compared to other groups (p <0,05). The calcium present in the bones of groups 2 and 8 did not defer significantly of the group control. The biological use of calcium did not defer between the animals of the groups control, 2 and 8, showing that the treatment in which there was used eggshell like a source of calcium was similar that in which the suplementation of calcium with vitamins D and K were done. When the suplementation of calcium from eggshell powder using vitamins D and K in basal levels, or without these vitamins was done, the biological use of calcium was low in comparison to control group. The results of hematological analysis, alkaline phosphatase and calcium serum of the groups studied, in most of all results, were similar to control group. The animals of the group 3 (diet 13 with calcium of the shell) were the only one who presented results to phosphorus serum that differed from the animals of control group.

AUTOR(ES)

DATA DE PUBLICAÇÃO

RESUMO

ASSUNTO(S)

ACESSO AO ARTIGO

Documentos Relacionados