Biological significance of carbohydrate chains on monoclonal antibodies.

AUTOR(ES)

Nose, M

RESUMO

We have prepared monoclonal hapten-specific mouse IgG2b antibodies depleted of asparagine-linked carbohydrate chains by treating the hybridoma cells with tunicamycin. The carbohydrate-deficient antibodies behaved in an identical manner to the normal antibodies with regard to fine antigen-binding reactivity (a Fab fragment feature) and protein A binding capacity [a feature requiring integrity at the CH2 and CH3 domain-interaction regions in the constant region of the heavy chain (CH)]. However, they lost the ability to activate complement, to bind to Fc receptors on macrophages, and to induce antibody-dependent cellular cytotoxicity. Furthermore, antigen-antibody complexes produced from such carbohydrate-deficient antibodies failed to be eliminated rapidly from the circulation. We conclude that removal of carbohydrate chains from IgG molecules may have a profound and highly select impact on the biological activity to these antibodies.

Documentos Relacionados

• Biological significance of Giardia-specific antibodies.
• Biological activities of antitrinitrophenyl and antidinitrophenyl mouse monoclonal antibodies.
• Restricted reassociation of heavy and light chains from hapten-specific monoclonal antibodies.
• Clinical significance and interpretation of antinuclear antibodies.
• Clinical laboratory applications of monoclonal antibodies.