Biological synthesis of the analgesic hydromorphone, an intermediate in the metabolism of morphine, by Pseudomonas putida M10.
AUTOR(ES)
Hailes, A M
RESUMO
The morphine alkaloid hydromorphone (dihydromorphinone) was identified as an intermediary metabolite in the degradation of morphine by Pseudomonas putida M10. A constitutive NADH-dependent morphinone reductase capable of catalyzing the reduction of the 7,8-unsaturated bond of morphinone and codeinone, yielding hydromorphone and hydrocodone, respectively, was shown to be present in cell extracts. The structures have been identified by 1H nuclear magnetic resonance and mass spectrometry. Morphinone reductase has been partially purified by anion-exchange and gel filtration chromatography. This enzyme has potential applications as a biocatalyst for the synthesis of the highly potent analgesic hydromorphone and the antitussive hydrocodone.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=182252Documentos Relacionados
- Transformations of morphine alkaloids by Pseudomonas putida M10.
- Plasmid Involvement in Acyclic Isoprenoid Metabolism by Pseudomonas putida
- Metabolism of and inhibition by chlorobenzoates in Pseudomonas putida P111.
- Metabolism of resorcinylic compounds by bacteria: orcinol pathway in Pseudomonas putida.
- Metabolism of Phenol and Cresols by Mutants of Pseudomonas putida
