Biosynthesis of Biotin in Microorganisms VI. Further Evidence for Desthiobiotin as a Precursor in Escherichia coli
AUTOR(ES)
Pai, Chik H.
RESUMO
Biotin auxotrophs were isolated from Escherichia coli K-12. One of the mutants was unable to grow on desthiobiotin and accumulated a large amount of a vitamer in medium when growing on an optimal concentration of biotin. The production of the vitamer was inhibited in the presence of an excess amount of biotin. The vitamer was identified as desthiobiotin on the basis of biological activities, avidin combinability, and chromatographic characteristics. The mutant lacked the ability to convert desthiobiotin to biotin. These results further support the hypothesis that desthiobiotin is a normal intermediate in the biosynthesis of biotin in E. coli.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=276924Documentos Relacionados
- Biosynthesis of membrane-bound nitrate reductase in Escherichia coli: evidence for a soluble precursor.
- Biosynthesis of Desthiobiotin in Cell-free Extracts of Escherichia coli1
- Metabolism of Biotin and Analogues of Biotin by Microorganisms IV. Degradation of Biotin, Oxybiotin, and Desthiobiotin by Lactobacillus casei
- Conversion of Desthiobiotin Into Biotin or Biotinlike Substances by Some Microorganisms1
- Repression of Biotin Biosynthesis in Escherichia coli During Growth on Biotin Vitamers