Biosynthesis of Glutamic Acid in Saccharomyces: Accumulation of Tricarboxylic Acid Cycle Intermediates in a Glutamate Auxotroph
AUTOR(ES)
Crocker, Wilson H.
RESUMO
Aconitaseless glutamic acid auxotroph MO-1-9B of Saccharomyces grew in glutamic acid-supplemented minimal medium, but failed to grow when glutamic acid was substituted by proline, arginine, ornithine, or glutamine. This mutant was also unable to utilize lactate or glycerol as a carbon source. Under a glutamic acid-limiting condition, by using acetate-1-14C as tracer, the mutant accumulated rather large amounts of 14C-citric acid and 14C-succinic acid when compared with the wild-type strain. Under excess glutamic acid supplementation, accumulation of citric acid and succinic acid was considerably reduced. When 14C-glutamic acid-(U) was used as tracer, 14C-α-ketoglutaric acid, 14C-citric acid, and 14C-succinic acid were accumulated in the mutant. The citric acid peak was the largest, followed by α-ketoglutaric acid and succinic acid. In the wild-type strain under similar conditions, only small amounts of 14C-citric acid and 14C-succinic acid and no 14C-α-ketoglutaric acid were accumulated.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=379779Documentos Relacionados
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