Biosynthesis of Vitamin B6 by a Yeast Mutant1
AUTOR(ES)
Pardini, R. S.
RESUMO
The gradient-plate technique was employed to isolate mutants of Saccharomyces marxianus (NRRL-Y-1550) which, when grown in a synthetic culture medium, excreted about 2 μg/ml of vitamin B6 as ascertained by microbiological assay. The major component that possessed vitamin B6 activity was isolated by ion-exchange column chromatography and identified as pyridoxol by ultraviolet and fluorescence spectroscopy, as well as by paper chromatography and various chemical tests. Pyridoxal was also identified as one of the excreted compounds. Two other compounds that possessed vitamin B6 activity were excreted in smaller quantities in the growth medium and have not yet been identified; they are not phosphates of vitamin B6. The amount of vitamin B6 excreted was not increased when the mutant was grown in the presence of various oxidation products of this vitamin. The methods and results reported here may be helpful in future studies on the biosynthesis of vitamin B6.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=252358Documentos Relacionados
- Biosynthesis of vitamin B6 by bacteria.
- Control of Vitamin B6 Biosynthesis in Escherichia coli1
- Vitamin B6 biosynthesis in higher plants
- Role of Vitamin B6 Biosynthetic Rate in the Study of Vitamin B6 Synthesis in Escherichia coli
- Identification of the Forms of Vitamin B6 Present in the Culture Media of “Vitamin B6 Control” Mutants
