BKV splice sequences based on analysis of preferred donor and acceptor sites.

AUTOR(ES)

Seif, I

RESUMO

We have determined the DNA sequences which correspond to the splicing regions in the transcripts of human papovavirus BKV, an evolutionary variant of SV40. To precisely localize the excision points in the BKV sequence, we have conducted a preliminary analysis of numerous viral and eukaryotic splice site sequences. This analysis suggests that the preferred sequence for the donor site belongs to at least one of four groups: Pu GTAxG, Pu GTAxxT, Pu GTxx GT, Pu GTxAG (= the cleavage site). These four groups derive from the two basic sequences, Pu GTxxG and Pu GTA. An optimal donor site might be: AG GTAAGT. The preferred sequence for the acceptor site is of the form PyPyxxPyAG; no dinucleotide AG occurs within 13 nucleotides prior to the terminal AG of the 3' end of the intervening sequences. As we have found in this study of BKV splicing RNA splice points when the DNA sequence is known.

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