Both cholera toxin-induced adenylate cyclase activation and cholera toxin biological activity are inhibited by antibodies against related synthetic peptides.
AUTOR(ES)
Jacob, C O
RESUMO
The immune response against six synthetic peptides corresponding to various segments of the B subunit of cholera toxin was evaluated. Conjugates in which the peptides were covalently linked to tetanus toxoid served for immunization of rabbits. As previously reported, four of these conjugates elicited antibodies cross-reactive with intact cholera toxin. We report here that antisera against two of these synthetic peptides inhibit the entire spectrum of activities of the intact cholera toxin. This is manifested both on the biochemical level (adenylate cyclase induction) and on the biological effect (intestinal fluid secretion). These results indicate that these peptides may serve as suitable candidates for preparation of a synthetic anticholera vaccine.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=392259Documentos Relacionados
- Role for Macrophages and Thymus-Derived Lymphocytes in Cholera Toxin-Induced Immunosuppression
- Reversal and inhibition of cholera toxin-induced secretion in isolated rabbit ileum.
- Monoclonal immunoglobulin A antibodies directed against cholera toxin prevent the toxin-induced chloride secretory response and block toxin binding to intestinal epithelial cells in vitro.
- Neutralization of Salmonella toxin-induced elongation of Chinese hamster ovary cells by cholera antitoxin.
- Mechanism of activation of adenylate cyclase by cholera toxin.