Calorie restriction extends yeast life span by lowering the level of NADH
AUTOR(ES)
Lin, Su-Ju
FONTE
Cold Spring Harbor Laboratory Press
RESUMO
Calorie restriction (CR) extends life span in a wide variety of species. Previously, we showed that calorie restriction increases the replicative life span in yeast by activating Sir2, a highly conserved NAD-dependent deacetylase. Here we test whether CR activates Sir2 by increasing the NAD/NADH ratio or by regulating the level of nicotinamide, a known inhibitor of Sir2. We show that CR decreases NADH levels, and that NADH is a competitive inhibitor of Sir2. A genetic intervention that specifically decreases NADH levels increases life span, validating the model that NADH regulates yeast longevity in response to CR.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=314267Documentos Relacionados
- High Osmolarity Extends Life Span in Saccharomyces cerevisiae by a Mechanism Related to Calorie Restriction
- Sir2-Independent Life Span Extension by Calorie Restriction in Yeast
- Assimilation of Endogenous Nicotinamide Riboside Is Essential for Calorie Restriction-mediated Life Span Extension in Saccharomyces cerevisiae*
- Hypoxia Extends the Life Span of Vascular Smooth Muscle Cells through Telomerase Activation
- Quantifying Yeast Chronological Life Span by Outgrowth of Aged Cells
