Canine model for the simultaneous measurement of antibiotic levels in tissues and bacterial killing rate.
AUTOR(ES)
Wagner, D S
RESUMO
Antibiotic levels in serum are commonly used to guide antibiotic therapy. The antibiotic levels in interstitial fluid are a more accurate reflection of the efficacy of antibiotic penetration into the tissues. Although there are experimental models for determining interstitial fluid levels, there is no model for measuring the in vivo killing of bacteria, which is the endpoint of antibiotic therapy. We developed an accurate, reliable animal model which allows measurement of the in vivo killing of bacteria along with a determination of antibiotic levels in tissues. Modified Sykes-Moore chambers were applied to the dissected external oblique muscle of 14 dogs. The chambers were inoculated with clinical isolates of Staphylococcus aureus or Escherichia coli. The dogs were treated with cefoxitin or gentamicin. Quantitative cultures were performed, and the antibiotic levels in interstitial fluid were determined.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=180484Documentos Relacionados
- Simultaneous measurement of bacterial flagellar rotation rate and swimming speed.
- Variation of ribosomal proteins with bacterial growth rate.
- Infinite allele model with varying mutation rate.
- Evaluation of data transformations used with the square root and schoolfield models for predicting bacterial growth rate.
- Evaluation of a 200 mm long vacuum aspiration tube for measurement of erythrocyte sedimentation rate.