Capacitance cytometry: Measuring biological cells one by one
AUTOR(ES)
Sohn, L. L.
FONTE
The National Academy of Sciences
RESUMO
Measuring the DNA content of eukaryotic cells is a fundamental task in biology and medicine. We have observed a linear relationship between the DNA content of eukaryotic cells and the change in capacitance that is evoked by the passage of individual cells across a 1-kHz electric field. This relationship is species-independent; consequently, we have developed a microfluidic technique—“capacitance cytometry”—that can be used to quantify the DNA content of single eukaryotic cells and to analyze the cell-cycle kinetics of populations of cells. Comparisons with standard flow cytometry demonstrate the sensitivity of this new technique.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=27083Documentos Relacionados
- Affordable CD4+-T-Cell Counting by Flow Cytometry: CD45 Gating for Volumetric Analysis
- Quantitative Detection of Hepadnavirus-Infected Lymphoid Cells by In Situ PCR Combined with Flow Cytometry: Implications for the Study of Occult Virus Persistence
- Large DNA fragment sizing by flow cytometry: application to the characterization of P1 artificial chromosome (PAC) clones.
- Platelet antibody detection by flow cytometry: an effective method to evaluate and give transfusional support in platelet refractoriness
- Detection of p53 in inflammatory tissue and lymphocytes using immunohistology and flow cytometry: a critical comment.