Caracterização biológica e molecular de amostras do Trypanosoma cruzi isoladas de escolares e de suas mães residentes no Vale do Jequitinhonha, MG.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

This study intended to explore the hypothesis of straight correlation between T. cruzi genetics and biological characteristics with the development of Chagas disease clinical status. For this, T. cruzi strains were isolated from two children and four child/mother pairs, from Berilo and José Gonçalves de Minas municipalities, Jequitinhonha Valley, MG. The clinical status of the patients was analyzed by clinical examination, electrocardogram, rays-X of thorax and esophagus contrasted. Morphobiological characterization of childrens strains was studied in groups of eight Swiss mice, inoculated with 10.000 blood trypomatigotes, via IP, being analyzed the parasitemia curve, pre-patent period, patent period, maximum peak of parasitemia, day of maximum of parasitemia, morphology of blood trypomastigotes and mortality. Following, successive blood passages of the strains were performed in these animals, inoculated at the same conditions, to verify their behaviour after this manipulation. Molecular characterization of all strains was performed by the analysis of isoenzymes, rDNA 24Sα, COXII gene and microssatelites profiles. Five children displayed the indeterminate clinical form of the disease and one cardiac moderate, whereas all mothers were cardiac with distinct clinical status. All childrens strains showed similar behavior in mice, being observed 100% of infection, low virulence, null mortality and predominance of large blood trypomastigotes, being these characteristics also observed after successive blood passages. Despite all strains presented the same genetic classification, the microssatelites analysis was able to reveal distinct profiles for each pair of strains child/mother, suggestive of congenital transmission. Although the microssatelites showed stronger discrimination power to segregate strains of same genetic classification as compared to other markers, there is no correlation between the microsatelites profile with the biological characteristics of the strains and clinical status of each pair child/mother. Results suggest that other elements such as host factors and/or the length of infection may play important role determining the clinical evolution of Chagas disease.

ASSUNTO(S)

parasitologia vale do jequitinhonha trypanosoma cruzi caracterização biológica e molecular características morfobiológicas

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