Caracterização de anticorpos monoclonais contra rotavírus bovino e suas aplicações como ferramenta de diagnóstico. / Characterization of monoclonal antibodies against bovine rotavirus and their diagnostic applications.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2005

RESUMO

Rotavirus, a member of the Reoviridae family, is the most important etiologic agent of diarrheas in bovine. Complete particules measure 70 nm in diameter, are nonenveloped and have a distinctive double-layered icosahedral protein capsid that consists of an outer and an inner layer. Within the inner capsid is a third layer, the core, that contains the virus genome consisting of 11 segments of double-strand RNA. The genome codes structural proteins VP1, 2, 3, 4, 6 e 7. The objective of this stydy was the characterization of monoclonal antibodies (MAbs) and the use of these MAbs as diagnosis tools by Isotyping, Dot-blot, Western-blot, immunofluorescence and ELISA techniques. The immunochemistry characterization showed that all were isotype IgG2a with a kappa light chain. The Dot-blot immunoassay showed that the MAbs 1G5, 4F7, 1E12, 4F3 detected viral antigens and only two MAbs (1E12 e 4F3) recognized viral proteins by Western-blot. The five MAbs reacted positively to indirecta immunofluorescence (IFI) and all MAbs can detect rotavirus antigen in fecal samples by indirect ELISA. We identificated two groups of Mabs: one with 4F7, 1E12 e 1G5 showing stimulant results by use detect rotavirus antigen in fecal samples and other with 4F3 e 3C12 by use detected Rotavirus antigens in cell culture by IFI. The results obtained discuss the potential use of these MAbs as diagnosis tools in diarrheas by Rotavirus in bovines.

ASSUNTO(S)

characterization caracterização rotavírus farmacia monoclonal antibodies anticorpos monoclonais rotavirus

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