Caracterização genética de Alouatta caraya (Primates, Atelidae) utilizando marcadores heterólogos do tipo microssatélites

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

Alouatta genus, usually known as howler-monkeys, is the most widely dispersed Neotropical monkeys. In the present work we describe the demographic, morphological and genetic characterization of an Alouatta caraya population from Porto Primavera, São Paulo, Brazil. The demographic characterizations were performed by the evaluation of the distribution of male and female in the entire population (N = 501) and between different age clusters: Adults, Subadults, Yong and Infants. Our results showed equal distribution of males and females when total population are considered (χ2 = 1,7786, GL = 1, p = 0,1823). The same were observed using test z to compare the distribution among clusters. We also evaluate body weight and total size between clusters. The body weight and total body size comparisons corroborate the described in literature: intense sexual dimorphism between Adults, lower between Young and absent between Infants. The genetic characterization was performed using heterologous microsatellite markers originally described to Alouatta belzebul. Genomic DNA of 100 specimens were isolated by a phenol-chlorophorm protocol and 20 markers were tested and amplified by PCR and genotyped in an ALFexpress TM II automated sequencer. Seven markers showed positive amplicons Ab04, Ab06, Ab07, Ab09, Ab10, Ab17 and Ab12 and were analyzed in Porto Primavera Alouatta caraya population, except Ab12. 66.67% (SD = 0.47) of the analyzed loci presented polymorphism. The number of alleles ranged between 1 17, and the average number of alleles by locus were 7.83 (SD = 6.85). Ab09 and Ab10 presented monomorphic to this population. The average heterozygosity observed were 0.52 (SD = 0.38) and the expected were 0.48 (SD = 0.42). All analyzed loci are in Hardy-Weinberg disequilibrium, except for Ab17 (p = 0.223). The genetic variability levels observed in the present work are relatively higher when compared to Neotropical primates and are similar to those reported to species with great dispersion capacity and elevated population densities.

ASSUNTO(S)

primatas alouatta caraya howler-monkey guariba (macaco) - genética alouatta caraya biologia animal howler-monkey, microsatélites cebídeo genetica animal

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