Caracterização microbiológica de indivíduos portadores de doença periodontal crônica e agressiva generalizada

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

The purpose of the present investigation was to analyze the subgingival microbial profile through the Checkerboard DNA-DNA Hybridization technique in a Brazilian population with chronic periodontitis and generalized aggressive periodontitis. To this end, 72 subjects were selected, being 30 with chronic periodontitis (ChP), 12 subjects with generalized aggressive periodontitis (GAgP) and 30 healthy (H). The analyzed clinical parameters were: probing pocket depth (PD-mm), clinical attachment level (AL-mm), presence or absence of visible dental plaque, gingival bleeding, bleeding on probing and suppuration. Samples of subgingival biofilm were collected from 9 mesial surfaces in the subjects of the group S and of 9 sites (shallow <3mm, medium 4-6mm and deep >7mm) in the individuals of the groups GAgP and ChP, being appraised later in relation to 38 bacterial species through the Checkerboard DNA-DNA Hybridization technique. In relation to the clinical parameters, the groups GAgP and ChP were homogeneous and they presented the highest average percentiles of PD and AL sites with SS and SUP in comparison to the healthy subjects. The group ChP demonstrated the highest percent of colonies with visible bacterial plaque, while the GAgP and H were similar. The group ChP presented medium levels of total counts of the analyzed species significantly higher (37.1 x 106) than the groups GAgP (21.7 x 106) and H (2.7 x 106). A. actinomycetemcomitans was found in high counts, proportions and the percentile of colonies colonized in subjects of the group GAgP when compared to the healthy subjects (p <0.001). The complexes orange and red represented the largest proportions in the group ChP (30.6% and 24.9%, respectively) and in the group GAgP (32.3% and 31.1%, respectively), however without significant statistical differences among the groups. On the other hand, the healthy subjects only presented 4.3% of red complex and the largest proportions (32.9%) of the species of Actinomyces in comparison with the groups ChP (17.7%) and AP (5.2%), is statistically similar (p>0.05). In conclusion, the subgingival microbiota of ChP and GAgP markedly differed from H subjects. Additionally, slight differences in the overall profile of colonization were observed between these two forms of disease.

ASSUNTO(S)

doença periodontal crônica chronic periodontitis microbiota subgengival doença periodontal agressiva subgingival microbiota aggressive periodontitis odontologia

ACESSO AO ARTIGO

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