Carbohydrate moieties of procollagen: incorporation of isotopically labeled mannose and glucosamine into propeptides of procollagen secreted by matrix-free chick embryo tendon cells.
AUTOR(ES)
Clark, C C
RESUMO
Cells obtained from chick embryo tendons incorporate isotopically labeled glucosamine and mannose into the pro-alpha1 and pro-alpha2 chains of procollagen as judged by sodium dodecyl sulfate-gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The label was further localized to the propeptides of pro-alpha1 and pro-alpha2 by its chromatographic behavior after digestion with bacterial collagenase or alpha-chymotrypsin. Carbohydrate analysis of isolated pro-alpha chains showed the presence of labeled galactosamine in addition to mannose and glucosamine. Resistance to mild alkaline hydrolysis suggested that greater than 90% of the oligosaccharide units are not linked to the propeptide backbone by either serine or threonine.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=335833Documentos Relacionados
- Direct Numerical Simulation of a Compressible Flow and Matrix-Free Analysis of its Instabilities over an Open Cavity
- Molecular cytochemistry: incorporation of fluorescently labeled actin into living cells.
- Effects of endogenous and exogenous inhibitors on the incorporation of labeled precursors into DNA by human mononuclear cells.
- Incorporation of [14C]Glucosamine and [14C]Mannose into Glycolipids and Glycoproteins in Cotyledons of Pisum sativum L. 1
- IMMUNOGLOBULIN SYNTHESIS AND SECRETION, V. INCORPORATION OF LEUCINE AND GLUCOSAMINE INTO IMMUNOGLOBULIN ON FREE AND BOUND POLYRIBOSOMES*