Catabolism of Porphobilinogen by Etiolated Barley Leaves 1

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RESUMO

When [2,4-14C]porphobilinogen (PBG) or [2 (aminomethyl),5-14C]PBG is administered to etiolated barley (Hordeum vulgare L. var. Larker) leaves in darkness, label becomes incorporated into CO2, organic and amino acids, sugars, lipids, and proteins during a 4-hour incubation. Less than 1% of the label, however, is incorporated into porphyrins. The rate of 14CO2 evolution from leaves fed [2,4-14C]PBG is strongly inhibited by anaerobiosis but is unaffected by aminooxyacetic acid, while the rate of 14CO2 evolution from [2(aminomethyl),5-14C]PBG is strongly inhibited by aminooxyacetic acid but is not affected by anaerobiosis.

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