Catalytic Accelerations of 1012-Fold by an Enzyme-Like Synthetic Polymer

AUTOR(ES)

Kiefer, Helen C.

RESUMO

A synthetic polymer, polyethyleneimine containing dodecyl (C12H25)-chains for binding sites and imidazole moieties for functional catalytic groups, catalyzes the hydrolysis of phenolic sulfate esters by a two-step mechanism resembling that of the corresponding natural enzyme. Quantitative rate studies give kinetic parameters that show that the polymer (synzyme) accelerates the rate 1012-fold compared to unbound imidazole, and 102-fold compared to a type IIA aryl sulfatase enzyme.

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