CD3-associated heterodimeric polypeptides on suppressor hybridomas define biologically active inhibitory cells.
AUTOR(ES)
Weiner, D B
RESUMO
We have investigated the relationship between CD3 expression and the suppressor T-cell function. We have isolated stable clonal cell lines of the F12.23 suppressor T-cell hybridoma that are either CD3+ or CD3-. These lines were subjected to functional assays including inhibition of in vivo hapten-specific delayed-type hypersensitivity responses, in vitro hapten-specific interleukin 2 responses, as well as hapten-specific cytotoxic T-lymphocyte assays. In all assays, the functional suppressor phenotype absolutely correlated with CD3 surface expression. Furthermore, we have immunoprecipitated heterodimeric proteins that share molecular features with some receptor polypeptides previously described. CD3 polypeptides found on the surface of suppressor T cells are phosphorylated after phorbol ester stimulation. Collectively these studies unambiguously define the suppressive supernatant function as a product of CD3+ receptor-bearing T cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=281908Documentos Relacionados
- Expression of CD3-associated antigen-binding receptors on suppressor T cells.
- Single amino acid changes that render human IFN-alpha 2 biologically active on mouse cells.
- Expression of biologically active bovine luteinizing hormone in Chinese hamster ovary cells.
- Expression of biologically active heterodimeric bovine follicle-stimulating hormone in milk of transgenic mice.
- Growth inhibitory and stimulatory effects of retinoic acid on murine 3T3 cells.