cDNA cloning with a retrovirus expression vector: generation of a pp60c-src cDNA clone.
AUTOR(ES)
Kaplan, P L
RESUMO
We used a murine retroviral expression vector, containing a genomic clone of the chicken c-src gene, a bacterial origin of replication, and a selectable marker, to remove 10 introns from the c-src gene. All 10 introns were removed accurately, and no mutations were introduced. The processed gene encoded a functional pp60c-src protein tyrosine kinase.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=254166Documentos Relacionados
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