cDNA fingerprinting of osteoprogenitor cells to isolate differentiation stage-specific genes.
AUTOR(ES)
Candeliere, G A
RESUMO
A cDNA fingerprinting strategy was developed to identify genes based on their differential expression pattern during osteoblast development. Preliminary biological and molecular staging of cDNA pools prepared by global amplification PCR allowed discrim-inating choices to be made in selection of expressed sequence tags (ESTs) to be isolated. Sequencing of selected ESTs confirmed that both known and novel genes can be isolated from any developmental stage of interest, e.g. from primitive progenitors, intermediate precursors or mature osteoblasts. EST expression provides insight into possible interrelated physiological functions and putative interacting molecules during differentiation. This method offers a functional genomics approach to isolate differentiation stage-specific genes in samples as small as a single cell.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=148289Documentos Relacionados
- Sin, a stage-specific repressor of cellular differentiation.
- Ovule development: identification of stage-specific and tissue-specific cDNAs.
- Stage-specific DNA methylation in a fungal plant pathogen.
- Lack of Stage-Specific Proteins in Coccoid Helicobacter pylori Cells
- The trypanosome spliced leader small RNA gene family: stage-specific modification of one of several similar dispersed genes.