Cellular Levels of trp RNA-Binding Attenuation Protein in Bacillus subtilis

AUTOR(ES)
FONTE

American Society for Microbiology

RESUMO

Expression of the Bacillus subtilis trp genes is negatively regulated by an 11-subunit trp RNA-binding attenuation protein (TRAP), which is activated to bind RNA by binding l-tryptophan. We used Western blotting to estimate that there are 200 to 400 TRAP 11-mer molecules per cell in cells grown in either minimal or rich medium.

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