Changes in Transfer Ribonucleic Acids Accompanying Encystment in Acanthamoeba castellanii

AUTOR(ES)

McMillen, Janis

RESUMO

Transfer ribonucleic acid (tRNA) from exponentially growing cells (trophozoites) and from precysts of Acanthamoeba castellanii were examined by reversed-phase column (RPC-2) chromatography. This system gave excellent resolution of isoaccepting species of tRNA. The tRNAs for 12 amino acids were studied. A comparison of trophozoite and precyst tRNA elution profiles revealed no apparent differences in the number of isoaccepting species of alanyl-, arginyl-, asparaginyl-, glycyl-, leucyl-, lysyl-, methionyl-, phenylalanyl-, tryptophanyl-, or valyl-tRNAs. Seryl-tRNAs from trophozoites were eluted as three components, whereas precyst seryl-tRNAs were eluted as only two components. Precharged trophozoite and precyst isoleucyl-tRNAs were both eluted as single components; however, post-chromatography charging of trophozoite tRNA resulted in three components of activity for tRNAIle and only one component for precyst tRNAIle. None of the observed changes could be attributed to differences in synthetases or to the presence of altered tRNA lacking the CCA terminus or partially degraded by nucleases. The possible significance of these observations is discussed.

Documentos Relacionados

• Changes in transfer ribonucleic acid population of Acanthamoeba castellanii during growth and encystment.
• Hydrodynamic changes accompanying the thermal denaturation of transfer ribonucleic acid.
• Predicted editing of additional transfer RNAs in Acanthamoeba castellanii mitochondria.
• Nucleotide sequences of Acanthamoeba castellanii 5S and 5.8S ribosomal ribonucleic acids: phylogenetic and comparative structural analyses.
• Changes in transfer ribonucleic acids of Bacillus subtilis during different growth phases