Characterization of a 70S polyuridylic acid polymerase isolated from foot-and-mouth disease virus-infected cells.
AUTOR(ES)
Polatnick, J
RESUMO
A polyuridylic acid polymerase complex isolated from foot-and-mouth disease virus-infected cells sedimented at 70S in a sucrose gradient and appeared in the exclusion volume of an agarose column whose molecular weight cutoff was 5 x 10(6). Phenol extraction of the complex yielded a heterogeneous band of virus-specific RNA and an apparently host cell-derived 4.5 to 5S RNA, both of which are essentially single stranded. Neither RNA served as a template in the cell-free enzyme reaction. Polyacrylamide gel analysis revealed five polypeptides with molecular weights of 50,000, 56,000, 60,000, 70,000, and 74,000 and with molar ratios of 1:2:2:1:1, respectively. Autoradiography showed P56 to be the only major virus-induced polypeptide; the other proteins are apparently of host cell origin. Electron microscopic examination suggested a cartwheel shape for the polymerase complex which was seen to dissociate as polyadenylic acid was added. Antibody previously shown to inhibit enzyme activity aggregated the 70S units.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=256699Documentos Relacionados
- Effect of Actinomycin D on Virus-induced Ribonucleic Acid Polymerase Formation in Foot-and-Mouth Disease Virus-Infected Baby Hamster Kidney Cells
- Isolation of a foot-and-mouth disease polyuridylic acid polymerase and its inhibition by antibody.
- Detection of foot-and-mouth disease virus-infected cattle by assessment of antibody response in oropharyngeal fluids.
- Electrophoretic Characterization of Foot-and-Mouth Disease Virus-Specific Ribonucleic Acid
- Further characterization of a protein kinase from foot-and-mouth disease virus.
