Characterization of a H+ Efflux from Suspension-cultured Plant Cells 1

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RESUMO

A readily assayed H+ efflux from sycamore (Acer pseudoplatanus), rye (Lolium perenne), and bean (Phaseolus vulgaris cultivars Red Kidney and Small White) suspension-cultured cells has been detected and partially characterized. The H+ efflux has been shown to require a source of energy, to be significantly stimulated by Na+ and Mg2+ but not by K+ and Ca2+, and to have a pH optimum at 7. The study of this H+ efflux was undertaken because the characteristics of auxin-induced growth and of H+-induced growth are sufficiently similar to suggest that a H+ efflux may be an intermediate in the mechanism of auxin-induced growth. However, the H+ efflux from these suspension-cultured cells was found to be insensitive to exogenously added hormones.

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