Characterization of a Photosynthesizing Reconstituted Spinach Chloroplast Preparation 1: REGULATION BY PRIMER, ADENYLATES, FERREDOXIN, AND PYRIDINE NUCLEOTIDES

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RESUMO

A particulate preparation (MgP) capable of photosynthetic CO2 assimilation without the addition of stromal protein was obtained by rupturing whole spinach (Spinacia oleracea var. America) chloroplasts in 15 millimolar MgCl2 buffered with Tricine at pH 8.5. This CO2 assimilation was dependent upon light, inorganic phosphate, ferredoxin, ADP, NAD or NADP, and primer. Excepting glycolate, the products of CO2 fixation by MgP were similar to those found with whole chloroplasts.

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