Characterization of a porcine genomic clone encoding a major histocompatibility antigen: expression in mouse L cells.
AUTOR(ES)
Singer, D S
RESUMO
A porcine genomic clone encoding a major histocompatibility, (MHC) antigen was isolated by direct screening of a swine genomic library with a heterologous human MHC cDNA probe. Mouse L cells transformed with DNA from the clone stably express swine MHC antigen. Pig alloantisera specifically lyse transformant but not control cell lines in a complement-mediated cytotoxicity assay. Direct immunoprecipitation of radiolabeled cellular protein from transformed lines by pig alloantiserum results in the coprecipitation of swine MHC heavy chain and mouse beta 2-microglobulin, demonstrating the association of heterologous subunits of MHC antigens.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=345981Documentos Relacionados
- Differential expression of porcine major histocompatibility DNA sequences introduced into mouse L cells.
- Expression of hybrid class I genes of the major histocompatibility complex in mouse L cells.
- cDNA clone coding for part of a mouse H-2d major histocompatibility antigen.
- Expression in L cells of transfected class I genes from the mouse major histocompatibility complex.
- Hormonal regulation of cell surface expression of the major histocompatibility antigen H-2Ld in transfected cells.
