Characterization of a Porcine Lung Epithelial Cell Line Suitable for Influenza Virus Studies
AUTOR(ES)
Seo, Sang Heui
FONTE
American Society for Microbiology
RESUMO
We established a porcine lung epithelial cell line designated St. Jude porcine lung cells (SJPL) and demonstrated that all tested influenza A and B viruses replicated in this cell line. The infectivity titers of most viruses in SJPL cells were comparable to or better than those in MDCK cells. The propagation of influenza viruses from clinical samples in SJPL cells did not lead to antigenic changes in the hemagglutinin molecule. The numbers of both Sia2-3Gal and Sia2-6Gal receptors on SJPL cells were greater than those on MDCK cells. Influenza virus infection of SJPL cells did not lead to apoptosis, as did infection of MDCK cells. No porcine endogenous retrovirus was detected in SJPL cells, and in contrast to MDCK cells, SJPL cells did not cause tumors in nude mice.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=114519Documentos Relacionados
- Characterization of a porcine kidney cell line resistant to influenza virus infection.
- Mink Lung Epithelial Cells: Unique Cell Line That Supports Influenza A and B Virus Replication
- Hamster cell line suitable for transfection assay of transforming genes.
- Use of a Clonal Line of Porcine Kidney Cell Cultures for Primary Isolation and Vaccine Studies with Adenoviruses
- Serial propagation of porcine group C rotavirus (pararotavirus) in a continuous cell line and characterization of the passaged virus.